Composite

Part:BBa_K1406001:Design

Designed by: Sierra Tackett   Group: iGEM14_WLC-Milwaukee   (2014-10-09)


pLAC promoter + strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 116
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We were hoping to make a high-expressing, directional insertion system in which we used enzymes which were compatible with the BioBrick standard and as an end product form a BioBrick.


Source

phage lamda

References